Analysis Note

ControlHT-29 cells

Application

Research CategoryCell Structure

Detect CAR using this Milli-Mark Anti-CAR-PE Antibody, clone RmcB validated for use in FC.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR′s biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.

Immunogen

Native human coxsackie virus-adenovirus receptor protein

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Unconjugated version Cat. No. 05-644 is known to react with Mouse and Rat.

Physical form

Purified mouse monoclonal IgG1 conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Protein G Purified

Quality

Evaluated by flow cytometry using HT-29 cells.

Specificity

Antibody recognizes CAR.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

40 kDa Calculated. Uniprot indicates multiple isoforms ranging from 9 to 40 kDa